As quatro primeiras equipes a disputar a ISL serão Aqua Centurions, Energy Standard, DC Trident e Cali Condors. Presença brasileira com Breno Correia e Larissa Oliveira integrantes da equipe italiana Aqua Centurions.
Equipes Europeias
| AQUA CENTURIONS | ENERGY STANDARD | ||
| Apostolos Christou | Freya Anderson | Simonas Bilis | Charlotte Bonnet |
| Santo Condorelli | Ilaria Bianchi | Anton Chupkov | Imogen Clark |
| Breno Correia | Georgia Bohl | Chad Le Clos | Georgia Davies |
| Laszlo Cseh | Elena Di LIddo | Max Litchfield | Femke Heemskerk |
| Fabio Scozzoli | Silvia Di Pietro | Florent Manaudou | Fantine Lesaffre |
| Luca Dotto | Franziska Hentke | Ben Proud | Penny Oleksiak |
| Kristian Gkolomeev | Sarah Kohler | Mykhailo Romanchuk | Kayla Sanchez |
| Philip Heintz | Kaylee McKeown | Evgeny Rylov | Emily Seebohm |
| Nicolo Martinenghi | Hannah Miley | Sergii Shevtsov | Anastasiya Shkurdai |
| Lidon Munoz | Ilya Shymanovich | Sarah Sjostrom | |
| Alessandro Miressi | Larissa Oliveira | Maxim Stupin | Kierra Smith |
| Matteo Rivolta | Margherita Panziera | Kregor Zirk | Rebecca Smith |
| Simone Sabbioni | Federica Pellegrini | ||
| Alba Vazquez | |||
Equipes americanas
| DC TRIDENT | CALI CONDORS | ||
| Zach Apple | Anika Apostalon | Bowen Becker | Signe Bro |
| Kevin Cordes | Emma Barksdale | Jack Cartwright | Mallory Comerford |
| Abrahm DeVine | Lisa Bratton | Caeleb Dressel | Kelsi Dahlia |
| Ian Finnerty | Annika Bruhn | Nic Fink | Hali Flickinger |
| Zane Grothe | Natalie Coughlin | Townley Haas | Molly Hannis |
| Zach Harting | Bethany Galat | Anton Ipsen | Natalie Hinds |
| Robert Howard | Sarah Gibson | Radosław Kawęcki | Shayna Jack* |
| Jay Litherland | Siobhán Haughey | Mitch Larkin | Lilly King |
| Cody Miller | Tristan Hollard | Kacper Majchrzak | Melanie Margalis |
| Giles Smith | Madison Kennedy | Justin Ress | Kylie Masse |
| Velimir Stjepanovic | Simona Kubová | John Shebat | Olivia Smoliga |
| Jérémy Stravius | Katie Ledecky | Jan Świtkowski | Ariarne Titmus |
| Andreas Vazaios | Leah Neale | Mark Szaranek | Kasia Wilk |
| Leiston Pickett | Andrew Wilson | Kelsey Wo | |
| Brianna Throssell |
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